Are you confused about the differences between HPLC vs UPLC analysis? In the past few years, HPLC and UPLC have become the most widely used methods for testing liquid pharmaceutical products.
They offer a more accurate, sensitive, and faster testing solution for your products. However, the two methods can get a little complicated, and the industry standards differ.
Curious to learn more? Then read on! In this article, we’ll cover everything you need to know about the two methods. That way, you can choose the right solution for your testing needs.
What Is UPLC?
A liquid that gets injected into a column containing packing material. The liquid is then transported through the column by a high-pressure pump.
The packing material in the column contains stationary phase molecules that interact with the molecules in the liquid sample. As the fluid sample moves through the column, the different molecules in the sample move through the column at different rates.
The molecules in the fluid sample get separated by the time that they take to pass through the column. The separated molecules are then detected by a detector, and the data gets collected and analyzed.
What Is HPLC?
A type of chromatography is used to separate and analyze compounds that are dissolved in solutions. HPLC is widely used in the pharmaceutical and food industries, as well as in environmental testing.
HPLC columns are made of various materials, such as silica, alumina, and cellulose, that are coated with a stationary phase. The mobile phase, which consists of a solvent or a mixture of solvents, moves the compounds through the column.
You can visit www.chromtech.com to check on HPLC column supplies for fast, reliable, and consistent results.
What Are Their Key Differences?
HPLC and UPLC have their key differences, making them each important in their own ways. HPLC is important because it is used in laboratories to purify and separate chemicals.
On the other hand, UPLC is for scientific research to identify and quantify unknowns in a sample. Here are the factors that affect their difference:
UPLC is faster and has a higher resolution than HPLC. UPLC pumps can also deliver higher pressures than HPLC pumps, resulting in faster analysis times.
HPLC is typically more expensive than UPLC, due to the higher initial investment cost and the need for more maintenance. UPLC is less expensive and requires less maintenance, which can help you with your cost efficiency in the long run.
HPLC requires more regular and thorough maintenance, as it is more susceptible to clogging and build-up. UPLC, on the other hand, requires less frequent maintenance as it is less likely to experience these issues.
Ease of Use
High-performance liquid chromatography is relatively slow and takes a long time to run a sample. Ultra-performance liquid chromatography (UPLC) is a newer technology that is much faster and more efficient than HPLC.
Defining HPLC vs UPLC Today
This article helped you understand the two chromatography methods, HPLC vs UPLC, and their key differences. HPLC is best for use with volatile solvents, while UPLSC is best for use with non-volatile solvents.
The resolution and speed of analysis are also different between the two methods. HPLC is typically better for high-resolution and fast analysis, while UPLSC generally is better for low-resolution and slow analysis.
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